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Registro Completo |
Biblioteca(s): |
Embrapa Pecuária Sul; Embrapa Uva e Vinho. |
Data corrente: |
27/12/2012 |
Data da última atualização: |
27/12/2012 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
BRUNETTO, G.; TRENTIN, G.; CERETTA, C. A.; GIROTTO, E.; LORENSINI, F.; MIOTTO, A.; MOSER, G. R. Z.; MELO, G. W. de. |
Afiliação: |
Gustavo Brunetto, UFSC; GUSTAVO TRENTIN, CPPSUL; Carlos Alberto Ceretta, UFSM; Eduardo Girotto, UFSM; Felipe Lorensini, UFSM; Alcione Miotto, UFSM; Glaucia Regina Zaferi Moser, UFSM; George Wellington de Melo, Embrapa Uva e Vinho. |
Título: |
Use of the SPAD-502 in estimating nitrogen content in leaves and grape yield in grapevines in soils with different texture. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
American Journal of Plant Sciences, Irvine, v. 3, n. 11, p. 1546-1561, Nov. 2012. |
Idioma: |
Inglês |
Conteúdo: |
The SPAD reading may be used in estimating total nitrogen content (N) in leaves and even in estimating grape yield in grapevines. The objective of this study was to estimate total N content in leaves and grape yield using the SPAD-502 in grapevines submitted to nitrogen fertilization in soils with clayey and sandy texture. In 2008, two experiments were installed in the Southern region of Brazil. In experiment 1, Cabernet Sauvignon grapevines were planted in a soil with clayey texture and with application of 10, 20, 40 and 80 kg·N·ha−1·year−1. In experiment 2, Cabernet Sauvignon grape- vines were planted in a soil with sandy texture and with the application of 0, 10, 15, 20, 40, 80 and 120 kg·N·ha−1·year−1. In the grapevines of the two experiments and during the period from 2008 to 2010, SPAD readings were made on leaves throughout the flowering period and at change in color of the berries using the portable chlorophyll meter Mi- nolta-SPAD-502. The leaves were collected, dried, ground and submitted to analysis of the total N content. In addition, grape yield per hectare was evaluated. The SPAD-502 readings estimated the total N content in flowering and at change in color of the berries in the Cabernet Sauvignon grapevines grown on soils with clayey texture and sandy texture, es- pecially in the first year of evaluation. However, the precision of the SPAD-502 readings is low, with there being no relationship between the SPAD-502 readings and grape yield. MenosThe SPAD reading may be used in estimating total nitrogen content (N) in leaves and even in estimating grape yield in grapevines. The objective of this study was to estimate total N content in leaves and grape yield using the SPAD-502 in grapevines submitted to nitrogen fertilization in soils with clayey and sandy texture. In 2008, two experiments were installed in the Southern region of Brazil. In experiment 1, Cabernet Sauvignon grapevines were planted in a soil with clayey texture and with application of 10, 20, 40 and 80 kg·N·ha−1·year−1. In experiment 2, Cabernet Sauvignon grape- vines were planted in a soil with sandy texture and with the application of 0, 10, 15, 20, 40, 80 and 120 kg·N·ha−1·year−1. In the grapevines of the two experiments and during the period from 2008 to 2010, SPAD readings were made on leaves throughout the flowering period and at change in color of the berries using the portable chlorophyll meter Mi- nolta-SPAD-502. The leaves were collected, dried, ground and submitted to analysis of the total N content. In addition, grape yield per hectare was evaluated. The SPAD-502 readings estimated the total N content in flowering and at change in color of the berries in the Cabernet Sauvignon grapevines grown on soils with clayey texture and sandy texture, es- pecially in the first year of evaluation. However, the precision of the SPAD-502 readings is low, with there being no relationship between the SPAD-502 readings and grape yiel... Mostrar Tudo |
Palavras-Chave: |
SPAD-502. |
Thesagro: |
Análise foliar; Estimativa; Nitrogênio; Nutrição vegetal; Uva; Viticultura. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/72817/1/TrentinAJPS.pdf
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Marc: |
LEADER 02359naa a2200289 a 4500 001 1943590 005 2012-12-27 008 2012 bl uuuu u00u1 u #d 100 1 $aBRUNETTO, G. 245 $aUse of the SPAD-502 in estimating nitrogen content in leaves and grape yield in grapevines in soils with different texture.$h[electronic resource] 260 $c2012 520 $aThe SPAD reading may be used in estimating total nitrogen content (N) in leaves and even in estimating grape yield in grapevines. The objective of this study was to estimate total N content in leaves and grape yield using the SPAD-502 in grapevines submitted to nitrogen fertilization in soils with clayey and sandy texture. In 2008, two experiments were installed in the Southern region of Brazil. In experiment 1, Cabernet Sauvignon grapevines were planted in a soil with clayey texture and with application of 10, 20, 40 and 80 kg·N·ha−1·year−1. In experiment 2, Cabernet Sauvignon grape- vines were planted in a soil with sandy texture and with the application of 0, 10, 15, 20, 40, 80 and 120 kg·N·ha−1·year−1. In the grapevines of the two experiments and during the period from 2008 to 2010, SPAD readings were made on leaves throughout the flowering period and at change in color of the berries using the portable chlorophyll meter Mi- nolta-SPAD-502. The leaves were collected, dried, ground and submitted to analysis of the total N content. In addition, grape yield per hectare was evaluated. The SPAD-502 readings estimated the total N content in flowering and at change in color of the berries in the Cabernet Sauvignon grapevines grown on soils with clayey texture and sandy texture, es- pecially in the first year of evaluation. However, the precision of the SPAD-502 readings is low, with there being no relationship between the SPAD-502 readings and grape yield. 650 $aAnálise foliar 650 $aEstimativa 650 $aNitrogênio 650 $aNutrição vegetal 650 $aUva 650 $aViticultura 653 $aSPAD-502 700 1 $aTRENTIN, G. 700 1 $aCERETTA, C. A. 700 1 $aGIROTTO, E. 700 1 $aLORENSINI, F. 700 1 $aMIOTTO, A. 700 1 $aMOSER, G. R. Z. 700 1 $aMELO, G. W. de 773 $tAmerican Journal of Plant Sciences, Irvine$gv. 3, n. 11, p. 1546-1561, Nov. 2012.
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Embrapa Pecuária Sul (CPPSUL) |
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Registro Completo
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
22/02/2016 |
Data da última atualização: |
05/12/2016 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
VIEIRA, D. D. S. S.; EMILIANIC, G.; MICHELOZZI, M.; CENTRITTO, M.; LUROE, F. S; MORILLON, R.; LORETO, F.; GESTEIRA, A. da S.; MASERTI, B. |
Afiliação: |
DAYSE DRIELLY SOUZA SANTANA VIEIRA, CNR-IPSP; GIOVANNI EMILIANIC, CNR-IVALSA; MARCO MICHELOZZI, CNR-IBBR; MAURO CENTRITTO; FRANÇOIS LUROE; RAPHAËL MORILLON, UMR AGAP-INRA; FRANCESCO LORETO, NR-DISBA; ABELMON DA SILVA GESTEIRA, CNPMF; BIANCAELENA MASERTI, CNR-IPSP. |
Título: |
Polyploidization alters constitutive content of volatile organic compounds (VOC) and improves membrane stability under water deficit in Volkamer lemon (Citrus limonia Osb.) leaves. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Environmental and Experimental Botany, n.126, p. 1-9, 2016. |
ISSN: |
0098-8472 |
Idioma: |
Inglês |
Conteúdo: |
In Citrus species chromosome doubling naturally occurs in somatic embryos and doubled diploid plants often show better adaptation to adverse environmental condition. To understand the molecular determinants of stress acclimation, we examined the response to water deficit in diploid (2VL) and doubled diploid (4VL) seedlings of Volkamer lemon (Citrus limonia Osb.) assessing the profile of constitutive volatile organic compound (VOC) in control and stressed conditions. Physiological parameters and leaf volatile compound profiles were measured during water deficit and 24 h after rehydration of plants to field capacity. Net photosynthesis and stomatal conductance were reduced in water stressed leaves, with no significant differences between 2VL and 4VL plants. Malondialdehyde concentration, a marker of lipid peroxidation of cellular membranes, was significantly more higher in stressed 2VL leaves than in 4VL. The blend of constitutive VOC was different in control leaves being oxygenated sesquiterpenoids more abundant in 2VL leaves, and monoterpenoids more abundant in 4VL leaves. Water deficit did not stimulate biosynthesis of terpenoids, whereas accumulation of trans- 2 hexenal, a green leaf volatile (GLV) synthesized after membrane denaturation, was observed in stressed leaves of 2VL leaves, but not in 4VL leaves. Semiquantitative PCR showed an increase of the expression of HPL, the gene encoding for hydroperoxidase lyase which catalyzes 2-hexenal formation, only in 2VL plants. The expression of the putative dehydration transcription factor DREB2A was also observed only in 2VL water stressed plants. This work shows that level of ploidy may alter constitutive content of GLV by Citrus, therefore likely affecting plants capacity of protection and interaction with other organisms. Whereas diploid and double diploid plants showed similar physiological responses to water deficit, a biochemical marker indicated that membranes of double diploid leaves were more resistant to the stress.These results provide intriguing MenosIn Citrus species chromosome doubling naturally occurs in somatic embryos and doubled diploid plants often show better adaptation to adverse environmental condition. To understand the molecular determinants of stress acclimation, we examined the response to water deficit in diploid (2VL) and doubled diploid (4VL) seedlings of Volkamer lemon (Citrus limonia Osb.) assessing the profile of constitutive volatile organic compound (VOC) in control and stressed conditions. Physiological parameters and leaf volatile compound profiles were measured during water deficit and 24 h after rehydration of plants to field capacity. Net photosynthesis and stomatal conductance were reduced in water stressed leaves, with no significant differences between 2VL and 4VL plants. Malondialdehyde concentration, a marker of lipid peroxidation of cellular membranes, was significantly more higher in stressed 2VL leaves than in 4VL. The blend of constitutive VOC was different in control leaves being oxygenated sesquiterpenoids more abundant in 2VL leaves, and monoterpenoids more abundant in 4VL leaves. Water deficit did not stimulate biosynthesis of terpenoids, whereas accumulation of trans- 2 hexenal, a green leaf volatile (GLV) synthesized after membrane denaturation, was observed in stressed leaves of 2VL leaves, but not in 4VL leaves. Semiquantitative PCR showed an increase of the expression of HPL, the gene encoding for hydroperoxidase lyase which catalyzes 2-hexenal formation, only in 2VL plants. T... Mostrar Tudo |
Thesagro: |
Fruta citríca. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02826naa a2200241 a 4500 001 2037897 005 2016-12-05 008 2016 bl uuuu u00u1 u #d 022 $a0098-8472 100 1 $aVIEIRA, D. D. S. S. 245 $aPolyploidization alters constitutive content of volatile organic compounds (VOC) and improves membrane stability under water deficit in Volkamer lemon (Citrus limonia Osb.) leaves.$h[electronic resource] 260 $c2016 520 $aIn Citrus species chromosome doubling naturally occurs in somatic embryos and doubled diploid plants often show better adaptation to adverse environmental condition. To understand the molecular determinants of stress acclimation, we examined the response to water deficit in diploid (2VL) and doubled diploid (4VL) seedlings of Volkamer lemon (Citrus limonia Osb.) assessing the profile of constitutive volatile organic compound (VOC) in control and stressed conditions. Physiological parameters and leaf volatile compound profiles were measured during water deficit and 24 h after rehydration of plants to field capacity. Net photosynthesis and stomatal conductance were reduced in water stressed leaves, with no significant differences between 2VL and 4VL plants. Malondialdehyde concentration, a marker of lipid peroxidation of cellular membranes, was significantly more higher in stressed 2VL leaves than in 4VL. The blend of constitutive VOC was different in control leaves being oxygenated sesquiterpenoids more abundant in 2VL leaves, and monoterpenoids more abundant in 4VL leaves. Water deficit did not stimulate biosynthesis of terpenoids, whereas accumulation of trans- 2 hexenal, a green leaf volatile (GLV) synthesized after membrane denaturation, was observed in stressed leaves of 2VL leaves, but not in 4VL leaves. Semiquantitative PCR showed an increase of the expression of HPL, the gene encoding for hydroperoxidase lyase which catalyzes 2-hexenal formation, only in 2VL plants. The expression of the putative dehydration transcription factor DREB2A was also observed only in 2VL water stressed plants. This work shows that level of ploidy may alter constitutive content of GLV by Citrus, therefore likely affecting plants capacity of protection and interaction with other organisms. Whereas diploid and double diploid plants showed similar physiological responses to water deficit, a biochemical marker indicated that membranes of double diploid leaves were more resistant to the stress.These results provide intriguing 650 $aFruta citríca 700 1 $aEMILIANIC, G. 700 1 $aMICHELOZZI, M. 700 1 $aCENTRITTO, M. 700 1 $aLUROE, F. S 700 1 $aMORILLON, R. 700 1 $aLORETO, F. 700 1 $aGESTEIRA, A. da S. 700 1 $aMASERTI, B. 773 $tEnvironmental and Experimental Botany$gn.126, p. 1-9, 2016.
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